Top-Down Protein Sequencing Service

In top-down proteomics analysis, intact protein molecular ions generated by ESI or MALDI are introduced into a mass analyzer and undergo gas-phase fragmentation. Top-down analysis facilitates the direct observation of C-terminal and N-terminal truncations and the differentiation of isomers through different sequences.

 

Kellie, J. F. et al. Mol Biosyst. 2010.

Figure 1. Top-down Protein Sequencing Analysis Service

 

Compared to the traditional Edman degradation method, MtoZ Biolabs offers top-down protein sequencing analysis services using MALDI ISD mass spectrometry technology, providing N-terminal and C-terminal sequencing services. Sequencing can be performed even when the terminals are modified, including N-terminal acetylation, N-terminal pyroglutamate, sequence truncation, and signal sequence closure. This analysis can also be used to characterize protein degradation and enzymatic cleavage.

 

Top-down sequence analysis can confirm 20 to 80 residues of the N-terminus or C-terminus of intact proteins.