Protein Profiling Service

Proteins serve as the primary functional components of cells. Investigating proteins allows for a deeper understanding of biological processes, disease onset, and drug mechanisms. Protein profiling analysis, also known as shotgun proteomics, systematically examines all protein expression, functions, and interactions within biological samples. The objective of protein profiling analysis is to identify as many proteins as possible within a sample. This is achieved by lysing, digesting, and separating protein samples, followed by detecting and identifying the separated peptide segments using high-resolution mass spectrometry. This process enables the determination of the proteins present in the sample. Protein profiling analysis permits the identification of all proteins within a sample, allowing for the simultaneous identification of hundreds to thousands of proteins.

 

MtoZ Biolabs’ one-stop service for protein profiling analysis relies on ultra-high-resolution mass spectrometry technology, utilizing Thermo Fisher's Orbitrap Fusion Lumos mass spectrometry platform in conjunction with nanoLC-MS/MS chromatography. This approach comprehensively identifies a vast array of proteins within entire cells, tissues, or complex mixed samples.

 

Analysis Workflow

1. Sample Preparation

Collection and extraction of proteins from biological samples, followed by concentration and desalting.

 

2. Separation and Purification

Separation of protein complex mixtures into individual or partially purified proteins.

 

3. Enzymatic Digestion

Proteins are cleaved into smaller peptide segments for mass spectrometry analysis.

 

4. Peptide Separation

Further separation of digested peptide segments via high-performance liquid chromatography (HPLC).

 

5. Mass Spectrometry Analysis

Acquisition of mass-to-charge ratio (m/z) information for peptide segments through mass spectrometry.

 

6. Data Processing and Identification

Processing of data from mass spectrometry analysis, followed by protein sequence identification, quantification, and modification through database matching.

 

7. Bioinformatics Analysis

Functional annotation of proteins, pathway analysis, protein interaction networks, etc., to elucidate changes in biological processes.

 

Service Advantages

1. Discovery of low-expression but Functionally Significant Proteins

2. Systematic Study of the Entire Proteome to Reveal Complex Regulatory Mechanisms in Biological Processes

3. Comprehensive Identification and Quantification of a Large Number of Proteins Within Entire Cells, Tissues, or Complex Mixed Samples in a Short Timeframe

4. Discovery and Investigation of Protein Biomarkers

 

Applications

1. Comparative Analysis of Protein Expression Differences Between Normal and Diseased Cells to Identify Drug Targets and Biomarkers

2. Construction of Proteomic Databases to Provide Resources for Subsequent Research

3. Identification and Quantification of Intracellular Proteins to Reveal Molecular Mechanisms of Biological Systems under Different Conditions

 

Sample Submission Requirements

 

 

Deliverables

In the technical report, MtoZ Biolabs will provide you with a detailed report including:

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Protein Profiling Analysis Details

4. Mass Spectrometry Images

5. Raw Data