Protein Full-Length Sequencing Service

Full-length sequence information is essential for studying the integrity and correct translation of biopharmaceuticals. In general, protein sequence analysis service provided by MtoZ Biolabs includes three steps: digestion of proteins into smaller peptide fragments; tandem LC-MS/MS analysis of peptides; MS/MS data analysis. Traditional protein sequencing only uses trypsin for digestion, which renders the final protein sequence coverage of around 60%. To ensure 100% protein sequence coverage, MtoZ Biolabs uses up to 6 kinds of protein enzymes for protein digestions, eliminating any missing peptides.

Analysis Workflow

Applications

1. Determination of Full-Length Sequence of Proteins/Peptides/Antibodies/Vaccines

2. Analysis of PTMs & Chemical Modifications on Protein

3. Verification of the Correct Translation of Recombinant Proteins

Sample Submission Requirements

Format	Gel bands, gel spots, and liquid samples are acceptable.
Quantity	Gel bands/spots that are visible to naked eyes are adequate for protein identification. For liquid samples, a total of 5-10 ug proteins are required.
Purity	Purity of liquid samples should be as high as possible. For liquid sample <10 ug, please avoid large amounts of detergent and salt ions, and note the buffer composition and the estimated amount of total proteins when submitting samples.
Note	All reagents/solvent used must be of the highest purity to reduce contaminating substances. Samples should be handled with extreme caution and always in clean condition. Any source that may introduce contaminating proteins should be eliminated.

Case Study

In this project, we analyzed the full-length sequence of an antibody product sent from one of our customers. To ensure 100% sequence coverage, we selected 4 enzymes for protein digestion, based on the predicted antibody sequence. The sequence mapping results showed 100% sequence coverage.