Paraffin Embedded Sample Proteomics Service

For mass spectrometry-based proteomic analysis, animal samples typically include fresh tissues, blood, urine, and cells. Paraffin-embedded (FFPE) samples, especially those used clinically, offer the advantages of well-documented medical histories, accurate diagnoses, and comprehensive clinical data. They can be stably stored at room temperature for extended periods, making them highly valuable for disease research. Therefore, proteomic research on FFPE samples is of great importance in understanding disease mechanisms, identifying biomarkers, and studying rare diseases.

 

MtoZ Biolabs uses Thermo Fisher's Orbitrap Fusion Lumos mass spectrometry platform combined with nanoLC chromatography to provide comprehensive technical solutions for FFPE sample proteomic analysis. Simply communicate your research objectives and send us your samples, and we'll handle the rest, including protein extraction, protease digestion, peptide separation, mass spectrometry analysis, raw mass spectrometry data processing, and bioinformatics analysis.

 

Analysis Workflow

 

Coscia, F. et al. J Pathol. 2020.

 

Service Advantages

• High Accuracy: QC CV as low as 25% for large projects
• High Throughput: More than 9000 proteins can be identified at once
• High Repeatability: Up to 90%
• Comprehensive Quantification: Quantitatively identify nearly all detectable molecules, covering low-abundance proteins/peptides
• Low Cost: Reduce the cost by more than 3 times

 

Sample Submission Requirements

 

 

Deliverables

In the technical report, MtoZ Biolabs will provide you with a detailed technical information, including:

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Mass Spectrometry Images

4. Raw Data

5. Bioinformatics Analysis