HPLC Protein Analysis Service

High performance liquid chromatography (HPLC) is a technique that utilizes chemical and physical interactions between the stationary phase (particles inside the chromatographic column) and the mobile phase (solvent) to achieve high-resolution and high-sensitivity qualitative and quantitative analysis of proteins. It holds great potential in various fields such as bioscience, drug development, and quality control. As the field of HPLC protein analysis progresses from the early experimental stage to modern diversified techniques, HPLC not only supports protein research but also helps elucidate the complexity of biological systems, identify new drug targets, and optimize biopharmaceutical production processes.

 

The primary modes of HPLC protein analysis include reversed-phase high-performance liquid chromatography (RP-HPLC), ion-exchange chromatography (IEX), size-exclusion chromatography (SEC), and affinity chromatography (AC). RP-HPLC separates proteins based on hydrophobic interactions, suitable for protein purity identification and peptide analysis. IEX separates proteins based on charge differences, enabling the determination of protein isoelectric points and charge distribution. SEC separates proteins based on molecular size, evaluating molecular weight, polymer, and subunit composition. AC achieves protein separation through specific biomolecular recognition and is commonly used to study protein-ligand interactions

 

Analysis Workflow

1. Sample Preparation

Necessary pre-treatments such as impurity removal, concentration, and desalination are performed on received samples to enhance the accuracy and repeatability of analysis.

 

2. HPLC Method Optimization

Based on protein characteristics and client requirements, appropriate chromatographic columns, mobile phases, and detector parameters are selected. Conditions such as flow rate, mobile phase composition, and temperature are set accordingly.

 

3. HPLC Analysis

Samples are analyzed using the optimized HPLC method, and chromatograms are recorded and analyzed to obtain information on protein separation, purity, concentration, etc.

 

4. Data Processing and Analysis

Dedicated software is used for peak identification, quantification, and identification of chromatographic data.

 

Service Advantages

1. High Resolution

HPLC technology offers high resolution, enabling accurate separation among multiple proteins, effectively distinguishing structurally similar or functionally related proteins.

 

2. High Sensitivity and Accuracy

It can detect protein concentrations as low as nanograms.

 

3. Good Reproducibility

High stability and reliability of experimental results.

 

4. Strong Resistance to Contamination

Tolerant to impurities in samples to a certain extent, reducing the purity requirements of samples.

 

5. Wide Applicability

By selecting suitable chromatographic columns and mobile phases, it can adapt to various types of biological samples such as serum, tissue extracts, cell lysates, etc., providing comprehensive services to clients.

 

6. Customization

Our technical staff can optimize analysis methods according to client requirements to improve separation efficiency and meet specific research objectives.

 

Applications

1. Biopharmaceuticals

Applied in drug research, production, and quality control to evaluate key parameters such as purity, activity, and stability of drug proteins.

 

2. Proteomics

Used for separation, identification, and quantification of proteomes, combined with mass spectrometry for high-throughput analysis.

 

3. Biomarker Research

Discovery and validation of potential biomarkers associated with diseases or physiological processes.

 

4. Food Safety and Quality Control

Detection of protein components, additives, contaminants, etc., in food to ensure safety and quality.

 

5. Disease Diagnosis

Analysis of protein changes in biological samples to aid in disease diagnosis and assessment.

 

Sample Submission Requirements

 

*Please avoid using reagents that may interfere with HPLC analysis, such as high concentrations of salts, acids, bases, dehydrating agents, etc. If necessary, we can provide sample processing for you.

 

Deliverables

In the technical report, MtoZ Biolabs will provide you with a detailed technical information, including:

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Protein HPLC Analysis

4. Mass Spectrometry Images

5. Raw Data